LAB PROTOCOLS
Neural tissue cell culture
- Isolation of spinal cord and DRG neuron
- Isolation of LMMP and myenteric plexus
- Enteric neuronal cell culture
- IPSC-derived neural tissue organoid
Viral package and gene delivery
- AAV and systemic delivery for CNS and PNS
- Lenti-viral package
Immune cell isolation and culture
- Bone marrow macrophage culture
- Bone marrow DC culture
- Neutrophil isolation from BM and periphery
- IEL and LP isolation for immune profiling
- ER-Hoxb8 system
Mouse models
- Food allergy model
- IL-4C
- DSS colitis model
- AIEC infection
- EAE model
- B16 melanoma model
- Prostate cancer xenografts
Immuno-assay
- Whole mount GI IHC and IF
Gut permeability and motility assay
- FITC dextran assay
- Carmine-red dye assay
- PAS staining
- Ussing chamber
CRISPR pooled screen
- Optical pooled screen
- IBD & Genome-wide CRISPR library screen
Spatial transcriptomics
- STARmap
- Visium
- Slide-seq
- Nanostring